Isolation, Characterization and Quantitation of Heparin Binding Proteins and PDC-109 Protein from Buffalo Bull Semen
نویسندگان
چکیده
An Fn-II type group of protein present in seminal plasma of ungulates viz. heparin binding proteins (HBP) and PDC-I09 are considered as a new super family, that modulate the fertilizing ability of sperm (McCauley et al., 1999). Attempts to isolate these proteins from buffalo seminal plasma have been less successful. Although presence of proteins having af nity to heparin-Sepharose or gelatin-Sepharose (Arangasamy 2003: Kumar, 2005) or PDC-109 like protein (Harshan, 2007) has been reported, no direct evidence for the presence of PDC-109 in buffalo seminal plasma exists. This paper describes isolation, characterization and quantitation of HBP and PDC-I09 protein using a modi ed protocol from buffalo bull semen.
منابع مشابه
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